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. 2022 Sep 21;8(38):eabo0987. doi: 10.1126/sciadv.abo0987

Fig. 7. PKM2 regulates TGF-β1 signaling in HFL1 and IPF fibroblasts.

Fig. 7.

(A to C) Western blot analysis of indicated proteins in HFL1 cells transfected with PKM2 siRNA for 48 hours and treated with TGF-β1 (5 ng/ml) for 1 hour (A) or 24 hours (B and C). (D) Immunoprecipitation of PKM2 with anti-PKM2 antibody in HFL1 cells. Western blot analysis of indicated proteins is shown. (E to G) Western blot analysis of indicated proteins in the IPF lung fibroblasts transfected with PKM2 siRNA for 48 hours and treated with TGF-β1 (5 ng/ml) for 1 hour (E) or 24 hours (F and G). (H) Immunoprecipitation of PKM2 with anti-PKM2 antibody in the IPF lung fibroblasts. Western blot analysis of indicated proteins is shown.