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. 2022 Sep 20;221(11):e202109137. doi: 10.1083/jcb.202109137

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© 2022 Laidlaw et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S5. — Ist1 localizations in vivo. (A) Indicated strains expressing Ist1^WT-GFP and Ist1^KR-GFP were imaged by Airyscan microscopy. (B) The percentage GFP signal in endosomal foci quantified as a percentage of total cellular fluorescence. (C) Ist1^KR-GFP mislocalizes to the nucleus (pink arrow or Hoechst stain) in indicated mutants. (D) Airyscan microscopy used to localize indicated fluorescent proteins in WT cells. Scale bar, 5 µm.