Figure 2.

Alterations in NO/superoxide balance, eicosanoid, TNFα and IL-1β gene expression in the aorta with the progression of HF in Tgαq*44 mice compared with age-matched FVB mice. NO production in the isolated aorta (n = 5–13) (A), ${\mathrm{O}}_2^{•-}$ production in aortic rings (n = 5–13) (B), basal production of eicosanoid (n = 5–6) 6-keto PGF_1α (C), PGE₂ (D), PGD₂ (E), and 15-HETE (F) detected in the effluent after 45 min of incubation of isolated aortic rings. TNF (G) and IL-1β (H) gene expression (n = 5–6) in the aorta in 4-, 8-, 10-, and 12-month-old Tgαq*44 mice vs. FVB controls. Normality was assessed using a Shapiro–Wilk test. Results are presented as box plots (median, Q1, Q3, whiskers indicate minimum/maximum). Q1 and Q3 indicate the 25th and 75th percentiles, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Tgαq*44 mice compared with age-matched FVB controls using two-way ANOVA with post hoc Sidak test.; IL-1β, interleukin-1β; NO, nitric oxide; TNFα, tumour necrosis factor alpha.