FIG. 4.

In vitro transcription of nhaA. The reactions were preformed using pGM42 (0.1 μg) as a template and E. coli ς⁷⁰-RNA polymerase holoenzyme (1 U) alone or in the presence of either purified NhaR (6), H-NS, or both. Equal aliquots were removed and used as templates for a primer extension assay as for Fig. 1. (A) Comparison between in vivo (wild-type) and in vitro transcription. (B) In vitro transcription in the absence or presence of His-tagged NhaR (400 ng), H-NS (40 ng), or both as indicated.