Figure 2.

Human bone marrow MSCs’ metabolic fitness defines matrix chemokine secretions. MSCs derived from nine independent donors were seeded at the indicated cell numbers in microwell plates. Ninety-six hours later, MTT assay was performed and optical density was measured. (A) Dose-dependent increase in optical density values is shown with mean and SD. (B) Optical density values of MTT assays were subjected to linear regression analysis with the corresponding chemokine levels from the appropriate wells to obtain R² values. Linear regression plots are organized based on the ranking of the degree of correlation. Data were transformed to logarithmic scale to fit the regression line. (C) Correlation coefficient values with appropriate range of 95% confidence intervals for each chemokine are shown. Linear regression analysis was performed in GraphPad Prism to get r, R², and P values.