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. 2022 Jul 26;11(9):971–986. doi: 10.1093/stcltm/szac050

Figure 7.

Figure 7.

Functionality of chemokines in modulating human bone marrow MSC’s inhibitory potential on T-cell proliferation. Control, CCL7, CXCL16, CCL2, CXCL6, CXCL1, and CXCL2 siRNA-transfected MSCs were cultured and supernatants were collected 72-hour post-transfection and assayed for appropriate chemokines. (A) Quantitative levels of CCL7, CXCL16, CCL2, CXCL6, CXCL1, and CXCL2 in the supernatants of control and appropriate siRNA-transfected MSCs are shown. (B) Control, IDO CCL7, CXCL16, CCL2, CXCL6, CXCL1, and CXCL2 siRNA-transfected MSCs were cultured with SEB-activated PBMCs. Four days post-culture, T-cell proliferation was measured in flow cytometry by Ki67 intracellular staining. Proliferation of T cells (%CD3+ Ki67+) in the presence and absence of siRNA-transfected MSCs is shown with (B) representative flow cytometry plots. (C) Cumulative data derived from three independent MSC donors are also shown with mean and SD. siRNA-transfected MSCs and PBMCs were cocultured in a ratio of 1:4 or 1:8.