TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Bacterial strains | ||
| P. denitrificans | ||
| Pd1222 | Rifr, Specr, enhanced conjugation frequencies | 8 |
| Pd0921 | Pd1222, mxaX::Km | 15 |
| Pd0841 | Pd1222, ΔmxaY | 35 |
| Pd0841.hf | Pd0841, homology fragment | This study |
| Pd0841hf.611 | Pd0841hf, pPr611 integrated | This study |
| Pd6721 | Pd1222, flhA::Km | 21 |
| Pd6621 | Pd1222, fghA::Km | 14 |
| Pd6121 | Pd1222, cycB::Km | 20 |
| Pd5021 | Pd1222, flhR::Km | This study |
| Pd5121 | Pd1222, flhS::Km | This study |
| Pd9234 | Pd0841, flhR::Km | This study |
| Pd9235 | Pd0841, flhS::Km | This study |
| Pd13 | Pd0841hf.611, flhR A633G | This study |
| Pd1222::Pr071 | Pd1222, pPr071 integrated | This study |
| Pd1222::Pr501 | Pd1222, pPr501 integrated | This study |
| Pd5021::Pr071 | Pd5021, pPr071 integrated | This study |
| Pd5021::Pr501 | Pd5021, pPr501 integrated | This study |
| E. coli | ||
| S17-1 | Smr pro r− m+ RP4-2 integrated (Tc::Mu)(Km::Tn7) | 24 |
| TG1 | supE hsdΔ5 thi Δ(lac-proAB) F′ (traD36 proAB lacIq lacZ ΔM15) | 22 |
| Plasmids | ||
| pUC4K | Kmr (Tn903) | 32 |
| pGRPd1 | oriV (ColE1) AmproriT Smr | 31 |
| pRK2020 | Tcr, pRK2013 Km::Tn10 | 9 |
| pGEM7 | Ampr | Promega |
| pUT.hf | Ampr, mini-Tn5 homology fragment, Kmr | This study |
| pUT.Km | Ampr, mini-Tn5, Kmr | 16 |
| pLOF.hf | Ampr, mini-Tn10 homology fragment, Kmr | 16 |
| pEG400 | IncP, Smr, Spr, pUC12mcs | 10 |
| pEG400Gm | pEG400 derivative, Gmr | This study |
| pSK4 | pEG400Gm derivative, pcycA-flhA | This study |
| pBK11 | Smr, SprlacZ-promoter probe vector | 16 |
| pBK16 | pBK11, with amber codons in aadA and lacZ | |
| pJR62.1 | 3′ xoxF-cycB | |
| pNH15 | 5′ part of mxaF, mxaZ, 5′ part of mxaY 2.7-kbp genomic EcoRI fragment in pUC13 | |
| pPr611 | pBK16, pcycB-lacZ | This study |
| pPr071 | pBK11, pmxaZ-lacZ | This study |
| pPr501 | pBK11, pflhR-lacZ | This study |
| pR11.1 | pLAFR3 derivative, 23-kbp, chromosomal fragment of P. denitrificans | This study |
| pRTd5021 | pGRPd1, flhR::Km | This study |
| pRTd5121 | pGRPd1, flhS::Km | This study |