TABLE 2.
Growth characteristics of wild type and regulatory mutants of P. denitrificansa
| Strain | Relevant genotype | Presence of growth on:
|
|||||
|---|---|---|---|---|---|---|---|
| CH3OH | CH3NH2 | Choline | Formate | H2CO2 | Succinate | ||
| Pd1222 | Wild type | + | + | + | + | + | + |
| Pd0841 | ΔmxaY | + | + | + | + | + | + |
| Pd13(pSK4) | ΔmxaY flhR− pcycA::flhA | − | − | + | + | + | + |
| Pd13 | ΔmxaY flhR | − | − | − | + | + | + |
| Pd5021 | flhR::Km | − | − | − | ND | ND | + |
| Pd5021(pSK4) | flhR::Km pcycA::flAh | − | − | + | ND | ND | + |
| Pd5121 | flhS::Km | − | − | − | ND | ND | + |
| Pd5121(pSK4) | flhS::KM pcycA::flhA | − | − | + | ND | ND | + |
| Pd9234 | ΔmxaY flhR::Km | − | − | − | ND | ND | + |
| Pd9234(pSK4) | ΔmxaY flhR::Km pcycA::flhA | − | − | + | ND | ND | + |
| Pd9235 | ΔmxaY flhS::Km | − | − | − | ND | ND | + |
| Pd9235(pSK4) | ΔmxaY flhS::Km pcycA::flhA | − | − | + | ND | ND | + |
a
Growth was determined on solid media after 48 h of incubation at 35°C (choline or succinate) or 96 h of incubation (methanol, methylamine, formate, H2-CO2). +, growth; −, no growth; ND, not determined.