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. 2022 Sep 7;609(7928):761–771. doi: 10.1038/s41586-022-05161-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, FOS staining of NTS/AP of saline and LPS (0.5mg/kg) treated animals three hours after injection. b, TRAP2 labeling in NTS/AP region of LPS-labeled animals. Gq-mCherry, purple; Fos, cyan; DAPI, blue. c, Representative images of TRAP2 labeling in NTS/AP region of LPS labeled (top) and saline labeled (bottom) with cre-dependent Gi-DREADDs. Top three panels show 4-hydroxytamoxifen labeling with LPS for three anterior-posterior coordinates in a single animal while bottom panel shows labeling with saline. hM4Di-mCherry, magenta. d–f, food intake (d), water intake (e), and movement (f) for 0.5mg/kg of LPS from Fig. 1 experiment to serve as reference. g,h, Core temperature (g) and BAT temperature (h) at four hours post CNO injection for reactivation of saline labeled (dark gray) and LPS labeled (dark cyan) neurons. Saline treatment is shown for core temperature in light gray and light cyan. Two-way ANOVA core temperature P < 1x10⁻⁷; unpaired t-test BAT temperature P < 1x10⁻⁷). ****P < 0.0001 and NS, not significant (P > 0.05). All error bars represent s.e.m. The brain illustration in a was created using BioRender.com.

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