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. 2022 Aug 9;609(7928):793–800. doi: 10.1038/s41586-022-05185-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — a. Flow chart representing data processing for the nsp7/8/9/12 complex. b. A representative micrograph of the nsp7/8/9/12 complex grids. c. Representative 2D classes generated by RELION 2D-classification. d. Gold-standard FSC curve (blue), and map-model FSC curve (red). Curves were generated by cryoSPARC and Phenix suite, respectively. e. Local resolution of the nsp7/8/9/12 complex calculated by RELION from final cryoSPARC half-maps. Position of nsp9 is indicated. f-h. Exemplary cryo-EM density (black mesh) on (f) Polymerase (Magenta), (g) NiRAN (green) and (h) nsp9 (gold). Note weaker density in the N-lobe of the kinase-like NiRAN domain (left panel of g, compare top to bottom of the image), and poor density in nsp9 (h), in areas not in direct contact with the NiRAN domain.