Fig. 3.

Measuring mitochondrial temperature by fluorescent thermosensors. a Cells are stained by fluorescent thermosensors and gradually heated. b Fluorescence intensity, measured by means of fluorescent microscopy at different temperatures will be recorded. c A plot of fluorescence intensity versus temperature will be drawn “Calibration plot.” d Uncoupler reagent (such as FCCP) will be added to induce mitochondrial uncoupling. e By means of fluorescence microscope, the fluorescence intensity of the cells will be measured by fluorescence microscopy and plotted (c) to conclude the cell temperature