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. Author manuscript; available in PMC: 2023 Sep 1.
Published in final edited form as: J Immunol. 2022 Aug 3:ji2100870. doi: 10.4049/jimmunol.2100870

Figure 2. Notch/ligand signals promote generation of CCR10+NK1.1+ ILC1s from hematopoietic stem cells in vitro.

Figure 2.

A) FC analysis of the expression of Notch1 (top) and Notch2 (bottom) on thymic CD3−NK1.1+ cells of adult Rag1−/− mice. B) FC analysis of the expression of CD127, CD11b, CCR10(EGFP) and CD103 on gated CD3−NK1.1+ cells (far left) generated from Rag1−/−CCR10+/EGFP BM progenitor cells cultured on OP9 and OP9-DL1 cells for 19 days. Gray areas in the CCR10(EGFP) and CD103 histograms are of WT progenitor cells and isotype control antibody staining respectively, serving as negative controls for EGFP and CD103. Representative of four experiments of cells analyzed on day 18-22 after culture.