FIGURE 4.

Inhibition of binding of CRP mutants to immobilized Aβ by fluid-phase Aβ. Aβ monomers (100 μg/ml) or Aβ fibrils (100 μg/ml) were mixed with CRP. The mixture of CRP and Aβ was then added to the wells coated with Aβ. Bound CRP was detected by using a rabbit polyclonal anti-CRP antibody and HRP-conjugated donkey anti-rabbit IgG. The OD of the developed color was read at 405 nm. Data shown are mean ± SEM of three experiments. The p values were determined by employing linear regression analysis of the slopes. For both CRP mutants, the differences between all the curves when compared to each other were statistically significant (p<0.05; not shown).