FIGURE 6.

Effects of CRP on the growth of pre-formed Aβ fibrils. The time course of fibrillation of Aβ peptides was monitored by ThT fluorescence in the absence or presence of 100 μg/ml CRP. CRP was added to Aβ 45 min after the fibrillation began in the microtiter plate which was incubated at 37°C throughout with shaking. Fluorescence was measured every 15 min. Results are plotted as mean a.u. ± SEM of three experiments. Statistical analysis was performed for the time period of 2 h to 3 h; p values were determined by taking the mean of all points and employing student unpaired t test. For clarity, only those differences are indicated (NS) where the difference was statistically not significant (p>0.05). The difference between all other curves when compared to each other was statistically significant (p<0.001; not shown).