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Effects of mutations on the Ca2+-site-dependent proteolytic cleavage of CRP. Reducing SDS-PAGE of CRP treated with protease in the absence and presence of 5 mM Ca2+ was run on a 4–20% polyacrylamide gradient gel. Bands were visualized by Coomassie brilliant blue staining. Protease alone is shown in lane 17. The m.w. markers are shown in lanes 1 and 18.
