Fig. 6. Wash-deficiency is associated with impaired ROS production and migration.

a Representative kinetic analysis of the production of superoxide anion by WT and Wash-cKO (Washc1^Δhaemo) neutrophils stimulated with PMA and measured by the cytochrome c reduction assay. Mean ± SEM, n = 3. b Quantitative analysis of superoxide anion production. Each symbol represents an individual mouse from 2 independent experiments. n = 6. Mean ± SEM. *p = 0.0166; ***p = 0.0002. One-way ANOVA, Tukey’s multiple comparisons. c Study of the production of extracellular reactive oxygen species by WT and Wash-cKO neutrophils as analyzed by chemiluminescence using the cell impermeant probe isoluminol. Mean ± SEM, n = 3. **p = 0.0193 (area under the curve), two-tailed unpaired Student’s t-test; d Study of the production of total reactive oxygen species by WT and Wash-cKO neutrophils as analyzed by chemiluminescence using the cell permeant probe luminol. Mean ± SEM, n = 3. e Analysis of wild-type and Wash-cKO neutrophil migration in response to CXCL1 (KC) and CXCL2. Mean ± SEM, n = 3. **p = 0.0012 and ****p < 0.0001. One-way ANOVA, Tukey’s multiple comparisons. f Analysis of the plasma membrane expression of the β₂ integrin subunit CD11b in wild-type and Wash-cKO neutrophils by flow cytometry. Each symbol corresponds to an individual mouse from 3 independent experiments. Mean ± SEM. n = 9 for all conditions except PMA (n = 6). ns, not significant. Source data are provided as a Source Data file.