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. 2022 Sep 21;7:109. doi: 10.1038/s41541-022-00536-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a C6/36 cells were infected with rMP-12, RVax-1, rMP12-GM50, or rMP12-∆NSm21/384 at MOI 0.01. Virus replication kinetics at 28 °C are shown. The graph represents the means ± standard deviations of triplicate wells (two-way ANOVA; *p < 0.05, **p < 0.01, vs. rMP-12, F = 13.47, df = 12). b Aedes aegypti were fed on blood meals containing medium (mock), rMP-12, rMP12-GM50, or RVax-1. Total RNA from abdomen, leg, or head/thorax/wing from three pools (n = 10 per pool) of mosquitoes at 14 dpi in each group were used for qRT-PCR analysis. Copy number of viral M segment RNA was measured by Taqman qRT-PCR using the standard curve for in vitro synthesized RVFV M segment RNA. Means ± standard errors of three different pools are shown in a scattered dot plot graph (two-way ANOVA: F = 2.178, df = 6).