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. 2022 Sep 21;7:109. doi: 10.1038/s41541-022-00536-3

Fig. 3. Viral antigen distributions in Aedes aegypti infected with rMP-12, rMP12-GM50, or RVax-1.

Fig. 3

Aedes aegypti were fed on blood meals containing medium (mock), rMP-12, rMP12-GM50, or RVax-1. Whole bodies were fixed with 10% neutral buffered formalin at 14 dpi. Immunohistochemistry using anti-RVFV N antibody are shown. Low magnification images are from mock-infected mosquitoes (a, b, e, f) or rMP-12-infected mosquitoes (b, d, g, h) under brightfield microscopy (a–d) or merged with TRITC fluorescent image (e–h). Positive signals are shown in magenta (brightfield) or red (TRITC). Bars represent 100 µm. High magnification brightfield images from rMP-12-infected (i–k), rMP12-GM50-infected (l–n), or RVax-1-infected mosquitoes (o–q) in head, thorax, or abdomen are shown as merged with TRITC fluorescent images, respectively. Bars represent 25 µm. Arrows in L and N images indicate RVFV N antigens. FB fat body, MG midgut, MT Malpighian tubules, SG salivary gland, Mu muscle, TG thoracic ganglion, CG cerebral ganglion, JO Johnston’s organ, OM ommatidia, OC oocyte, OV ovaries.