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. 2022 Sep 21;5:1002. doi: 10.1038/s42003-022-03945-1

Fig. 1. IDH2 deficiency increases fat mass and sensitivity to high-fat diet-induced obesity in NAFLD mice with inflammation of the liver.

Fig. 1

a Body weights of male mice fed a normal diet (ND, black-WT, green-KO) and high-fat diet (HFD, blue-WT, red-KO) for 1–12 weeks. b Photographs of WT and KO fed an ND or HFD. c Epididymal fat mass. d–f Blood parameters. Data are means ± SEM of three independent experiments. *p < 0.05 vs. WT-ND mice. #p < 0.05 vs. WT-HFD mice. g Representative H&E-stained high-magnification images of mouse livers. h Representative immunohistochemical images of CD68 in the liver of WT and KO mice fed an ND or HFD; scale bars, 50 μm. i Western blot analysis of CD68 in the liver tissues of two mice per group. j–l Expression of IL-1β (j), IL-6 (k), and TNF-α (l) by quantitative real-time PCR, normalized to GAPDH. Data are means ± SEM of three independent experiments (n = 3). *p < 0.05 vs. WT-ND mice. #p < 0.05 vs. WT-HFD mice.