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. 2022 Sep 21;5:1002. doi: 10.1038/s42003-022-03945-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Mechanisms of fatty acid and cholesterol synthesis. b, c Citrate and acetyl-CoA levels in liver tissue determined using a colorimetric/fluorometric assay kit. d, e Expression of genes related to cholesterol efflux and synthesis, and fatty acid synthesis and β-oxidation, revealed by quantitative real-time PCR (normalized to GAPDH). Data are means ± SEM of three independent experiments (n = 3). *p < 0.05 vs. WT-ND mice. #p < 0.05 vs. WT-HFD mice.