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. 2022 Sep 21;5:1001. doi: 10.1038/s42003-022-03972-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Immunoblotting using anti-mouse FADS2 antibody of samples from testes and Leydig cells from FADS2^+/+ (WT) and FADS2^−/− (KO) mice and MA-10 cells. β-actin was used as a loading control. b–d Quantification of HUFA species in MA-10 cells. The values of each molecular species were expressed as a percentage relative to CTL. n = 3 for each group. The detailed lipidomics data are shown in Supplementary Fig. 4. e Reduced steroid hormone production in MA-10 cells treated with SC-26196. Steroid hormones were extracted from the supernatant. n = 4 for each group. Significance is based on unpaired two-tailed t-test with Welch’s correction. f Steroid hormone production in MA-10 cells supplemented with HUFAs. Steroid hormones were extracted from the supernatant. n = 4 for each group. Significance is based on Dunnett’s multiple comparisons test. Data shown are the mean ± SEM. Steroid hormone levels in the cell pellets are shown in Supplementary Fig. 4f. CTL control, hCG human chorionic gonadotropin, ARA arachidonic acid, EPA eicosapentaenoic acid, DHA docosahexaenoic acid, DPA n-6 docosapentaenoic acid.