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. 2022 Sep 21;5:1001. doi: 10.1038/s42003-022-03972-y

Fig. 4. Omega-6 HUFAs are required for steroid hormone production in Leydig cells.

Fig. 4

a–c Quantification of HUFA species in Leydig cells from FADS2+/+ (WT) and FADS2−/− (KO) mice. n = 3 for each group. The detailed lipidomics data are shown in Supplementary Fig. 5. d Reduced steroid hormone production in Leydig cells from FADS2−/− mice. Steroid hormones were extracted from the supernatant. n = 4 for each group. Significance is based on unpaired two-tailed t-test with Welch’s correction. e–g Steroid hormone production in Leydig cells from FADS2−/− mice supplemented with ARA (e), DPAn-6 (f), or DHA (g). n = 4 for each group. Significance is based on Tukey’s multiple comparisons test. Data shown are the mean ± SEM. Steroid hormone levels in the cell pellets were shown in Supplementary Fig. 5f–h. DHT dihydrotestosterone, hCG human chorionic gonadotropin, ARA arachidonic acid, DHA docosahexaenoic acid, DPA n-6 docosapentaenoic acid.