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. 2022 Sep 21;5:1001. doi: 10.1038/s42003-022-03972-y

Fig. 5. HUFA-containing cholesteryl ester species are preferred substrates for HSL.

Fig. 5

a The lipase activity of recombinant mouse hormone-sensitive lipase (mHSL). HEK293T cells were transfected with empty vector (mock) or mHSL-expressing vector (mHSL) and the cell lysate (1 mg/mL) was incubated with each cholesterol ester species (100 μM) for 30 min at 37 °C. FFA, free fatty acid. n = 3 for each group. b HSL-dependent FFA liberation upon human chorionic gonadotropin (hCG) stimulation in MA-10 cells. Triacsin C was pretreated to block the conversion of FFAs to fatty acyl-CoAs. The FFA levels insensitive to HSL-IN-1 were subtracted to evaluate HSL-dependent FFA production. n = 3 for each group. Significance is based on unpaired two-tailed t-test with Welch’s correction. Data shown are the mean ± SEM. The detailed data are shown in Supplementary Fig. 13.