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Substrate specificity of NagI GDO
| Substrate | Km (μM) | Relative activitya |
|---|---|---|
| Gentisate | 22.4 | 100 |
| 3-Methylgentisate | 10.7 | 63 |
| 3-Bromogentisate | 5.3 | 25 |
| 3-Isopropylgentisate | NDb | 76 |
Activity relative to that on gentisate (set at 100) measured at 100 μM for each substrate. The specific activity of the extract of E. coli(pWWF19-25) was 2.52 U/mg.
ND, not determined.
