Table 4.
Crossover analysis in assessing the gene-environmental interaction between the MTHFR gene and maternal folic acid supplementation for risk of CHD.
| MTHFR genotype | Maternal folic acid use | Number of controls | Number of cases | Adjusted OR (95%CI)† | P | FDR-P |
|---|---|---|---|---|---|---|
| rs2274976 | ||||||
| Wild genotype (CC) | Yes | 489 | 409 | 1.00 (reference) | ||
| Variant genotype (CT + TT) | Yes | 88 | 117 | 1.29 (0.91–1.83) | 0.157 | 0.235 |
| Wild genotype (CC) | No | 33 | 72 | 1.25 (0.76–2.06) | 0.376 | 0.752 |
| Variant genotype (CT + TT) | No | 10 | 22 | 1.03 (0.42–2.49) | 0.955 | 0.955 |
| rs4846052 | ||||||
| Wild genotype (CC) | Yes | 469 | 384 | 1.00 (reference) | ||
| Variant genotype (CT + TT) | Yes | 105 | 123 | 1.18 (0.84–1.65) | 0.339 | 0.339 |
| Wild genotype (CC) | No | 33 | 63 | 1.13 (0.68–1.86) | 0.643 | 0.772 |
| Variant genotype (CT + TT) | No | 10 | 22 | 1.36 (0.58–3.22) | 0.481 | 0.577 |
| rs1476413 | ||||||
| Wild genotype (CC) | Yes | 404 | 304 | 1.00 (reference) | ||
| Variant genotype (CT + TT) | Yes | 170 | 203 | 1.37 (0.96–1.83) | 0.030 | 0.060 |
| Wild genotype (CC) | No | 33 | 45 | 0.87 (0.51–1.63) | 0.625 | 0.938 |
| Variant genotype (CT + TT) | No | 10 | 40 | 2.66 (1.21–5.85) | 0.015 | 0.023 |
| rs2066470 | ||||||
| Wild genotype (GG) | Yes | 477 | 362 | 1.00 (reference) | ||
| Variant genotype (GA + AA) | Yes | 97 | 145 | 1.83 (1.32–2.54) | 0.013 | 0.037 |
| Wild genotype (GG) | No | 35 | 61 | 2.42 (1.49–3.93) | 0.007 | 0.021 |
| Variant genotype (GA + AA) | No | 8 | 24 | 3.48 (1.37–8.83) | 0.009 | 0.027 |
| rs1801133 | ||||||
| Wild genotype (GG) | Yes | 263 | 220 | 1.00 (reference) | ||
| Variant genotype (GA + AA) | Yes | 311 | 287 | 1.21 (0.92–1.60) | 0.170 | 0.204 |
| Wild genotype (GG) | No | 20 | 31 | 1.69 (0.85–3.35) | 0.133 | 0.399 |
| Variant genotype (GA + AA) | No | 23 | 54 | 3.27 (1.85–5.78) | 0.000 | 0.000 |
| rs1801131 | ||||||
| Wild genotype (TT) | Yes | 424 | 295 | 1.00 (reference) | ||
| Variant genotype (TG + GG) | Yes | 150 | 212 | 1.89 (1.39–2.58) | 0.000 | 0.000 |
| Wild genotype (TT) | No | 33 | 38 | 0.97 (0.53–1.76) | 0.910 | 0.999 |
| Variant genotype (TG + GG) | No | 10 | 47 | 2.85 (1.29–6.29) | 0.009 | 0.018 |
CHD, congenital heart disease; CI, confidence interval; OR, odds ratio; FDR, false discovery rate; MTHFR, Methylenetetrahydrofolate reductase.
†
Adjusted for gender, maternal age at pregnancy onset, education status, residence location, adverse pregnancy history (induced abortion or labor, fetal death or stillbirth, neonatal death, hypertension of pregnancy, gestational diabetes mellitus), family history (consanguineous marriages), maternal lifestyle before this pregnancy, harmful chemical exposure history in this pregnancy.
Bold font indicates statistical significant.