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. 2022 Sep 8;13:920044. doi: 10.3389/fphys.2022.920044

FIGURE 2.

FIGURE 2

TNBS-induced colitis increased the P2X3 expression in the distal colon. (A–B) Western blotting showed that TNBS-induced colitis significantly increased the protein expression of the P2X3 receptor in the distal colon. n = 3 for each group. (C–D) P2X3 immunoreactivity in the submucosal plexus of the distal colon showed a significant increase in colitis at days 3 and 7 post-TNBS compared to the control group. Bar = 100 μm; n = 4 for each group. (E–F) P2X3 immunoreactivity in the muscular layer of the distal colon showed that TNBS-induced colitis significantly increased P2X3 expression compared to the control group. Bar = 50 μm; n = 4 for each group. *p < 0.05 and **p < 0.01 by one-way ANOVA followed by Newman–Keuls post hoc tests.