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. 2022 Sep 8;12:987692. doi: 10.3389/fcimb.2022.987692

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Copyright © 2022 Liu, Sun, Ying, Liu, Wang, Sun, Wang, Yang, Hui, Zhou, Hou and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathological staining and mNGS results in P1 and P5. Lymph node enlargement of the right axilla was seen in P1 (A). Confirmation of T. marneffei-specific amplification from lymph node tissue by mNGS showed 108,380 unique sequence reads of T. marneffei, accounting for 18.93% of the genome coverage (B). Granulomatous inflammation observed during histopathological examination of the cervical lymph node (C). PAS staining of the cervical lymph node revealed fungal spores (arrows) (magnification × 400) (D). A large number of neutrophil infiltrates were observed in the histopathological examination of abdominal lymph nodes of P5, and a patchy distribution of tissue cells was observed. Fungal spore-like substances were scattered or clustered in some tissue cells (magnification × 400) (E). PAS staining of the abdominal lymph node tissues revealed fungal spores (arrows) (magnification × 400) (F).