Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 8;9:965645. doi: 10.3389/fmolb.2022.965645

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Hamitouche and Jonic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Graphical summary of HEMNMA steps. (A) Input EM map or atomic structure (the reference) and input single particle images. (B) Normal mode analysis of the reference and selection of normal modes (vectors). (C) Elastic and rigid-body alignment of each single particle image with the reference using the selected normal modes. (D) Mapping of single particle images onto a low-dimensional (here, 2D) conformational space in which the reference can be animated (denser regions are marked with a darker red color; close points correspond to images with similar conformations and distant points to images with different conformations). (E) 3D reconstructions from the densest areas in the low-dimensional conformational space shown by squares in (D).