FIG. 2.
The O2R element is present in the −356 to −307 region of the OLE1 promoter. rAPase activity in cells of the wild-type strain (SH5143) harboring the respective reporter genes cultivated aerobically (+O2) or anaerobically (−O2) in YPDA medium was measured as described previously (48). Numbers indicate the position relative to the first nucleotide of the initiation codon (+1) of OLE1. PHO84 promoter sequences (nt −272 to −1) contain binding site E (nt −262 to −257) for the transactivator Pho4p and two putative TATA boxes (nt −122 and −99). Shaded boxes, structural regions of PHO5. The ANB1p-PHO5 reporter gene was used to verify anaerobic conditions. Error bars, standard deviations determined from a minimum of three independent measurements. The actual values of rAPase activity with their standard deviations are indicated. Other symbols are the same as those described for Fig. 1B.