TABLE 1.
S. cerevisiae strains used
| Strain | Genotypea | Source |
|---|---|---|
| SH4041 | MATa ura3 trp1 leu2-3,112 his3Δ pho3-1 pho5-1 | Our laboratory |
| SH5128 | MATa ura3 trp1 leu2-3,112 his3Δ pho3-1 pho5-1 rox1::LEU2 | This study |
| SH5420 | MATa ura3::[URA3 OLE1p-PHO5] trp1 leu2-3,112 his3Δ pho3-1 pho5-1 | This study |
| SH5421 | MATa ura3::[URA3 OLE1p-PHO5] trp1 leu2-3,112 his3Δ pho3-1 pho5-1 rox1::LEU2 | This study |
| SH5143 | MATα ura3-52 leu2-3,112 his4-519 trp1 pho3-1 pho5-1 canr | Our laboratory |
| SH5141 | MATα ura3-52::[URA3 OLE1p-PHO5] trp1 leu2-3,112 his4-519 pho3-1 pho5-1 canr | This study |
a
ura3::[URA3 OLE1p-PHO5] and ura3-52::[URA3 OLE1p-PHO5] represent the integration of the OLE1p-PHO5 fusion reporter gene into the ura3 locus using the plasmid p1166 as described in Materials and Methods. Other symbols are as described previously (32).