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Bacterial accumulation and α diversity of oral microorganisms in the orthodontic wire ligation model. Bacterial colony numbers from oral cavities under (A–C) aerobic and (D–F) anaerobic conditions. (G) Rarefaction curves for the oral bacterial communities. (H) Shannon diversity index for the orthodontic wire ligature groups. Data represent 3 independent experiments (n = 6). ns, not significant, *P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
