Table 1.
Gene | Primer sequences |
---|---|
Il1β | Sense primer: 5′-TCGCAGCAGCACATCAACAAGAG-3′ Anti-sense primer: 5′-AGGTCCACGGGAAAGACACAGG-3′ |
Tnf-α | Sense primer: 5′-GCCTCTTCTCATTCCTGCTTGTGG-3′ Anti-sense primer: 5′-GTGGTTTGTGAGTGTGAGGGTCTG-3′ |
Il6 | Sense primer: 5′-CTTCTTGGGACTGATGCTGGTGAC-3′ Anti-sense primer: 5′-AGGTCTGTTGGGAGTGGTATCCTC-3′ |
RunX2 | Sense primer: 5′-TCCCAGGCAGGCACAGTCTTC-3′ Anti-sense primer: 5′-AGCGGCGTGGTGGAGTGG-3 |
Alp | Sense primer:5′-CACGGCGTCCATGAGCAGAAC-3′ Anti-sense primer: 5′-CAGGCACAGTGGTCAAGGTTGG-3 |
RANKL | Sense primer:5′-ATGGAAGGCTCATGGTTGGATGTG-3′ Anti-sense primer: 5′-TGGCAGCATTGATGGTGAGGTG-3′ |
Opg | Sense primer:5′-TGCACAGTGAGGAGGAAGACATTG-3′ Anti-sense primer: 5′-CACTCCTGCTTCACGGACTGC-3′ |
Ocn | Sense primer:5′-AAGCCTTCATGTCCAAGCAGGAG-3′ Anti-sense primer: 5′-CGGTCTTCAAGCCATACTGGTCTG-3′ |
Gapdh | Sense primer: 5′-TGGAAAGCTGGGCGTGATG-3′ Anti-sense primer:5′-TACTTGGCAGGTTTCTCCAGG-3′ |