Table 1.

Overview of the catalytic properties of PAA pathway.

Protein	Organism	Substrate	Temperature/°C	Km/μM	Kcat/min−1	Vmax/Umg−1	References
BcePaaK1	Burkholderia cenocepacia	Phenylacetic acid		62	250		Law and Boulanger, 2011
BcePaaK2				150	300
TthPaaK	Thermus thermophilus	Phenylacetate	75	6	1,200	24	Erb et al., 2008
		CoA		30	1,200
		ATP		50	1,200
Py2PaaABCE	Pseudomonas sp. Y2	Phenylacetyl-CoA	30	1			Teufel et al., 2010
		NADPH		23		1	Teufel et al., 2012
		NADH				0.05
		Phenylacetyl-CoA		6
		O2		3
		Epoxyphenylacety-CoA		17
TthPaaG	Thermus thermophilus	1,2-epoxyphenyl- acetyl-CoA				138	Spieker et al., 2019
Py2PaaG	Pseudomonas sp. Y2					182
		Trans-3,4-didehydroadi poyl-CoA		564	0.21
TthPaaG	Thermus thermophilus			142	3.6
Py2PaaZ (hydrolysis)	Pseudomonas sp. Y2	Oxepin-CoA	22	35		7.6	Teufel et al., 2011, 2012
	Escherichia coli
EcoPaaZ (dehydrogenase)				20		33	Teufel et al., 2011
				11		32
		NADP+		56
Py2PaaI	Pseudomonas sp. Y2	Phenylacetyl-CoA	22			1.6	Teufel et al., 2012
EcoPaaI	Escherichia coli
AevPaaI	Aromatoleum evansii DSM6898		25	9.6	24.6		Song et al., 2006
			25	390	222
SphPaaI	Streptococcus pheumoniae	Phenylacetyl-CoA		90	390		Khandokar et al., 2016
		Decanoyl/C10-CoA		183	1968
Py2PaaY	Pseudomonas sp. Y2	2-hydroxycyclohepta- 1,4,6-triene-1- carboxyl-CoA	22	35		7.6	Teufel et al., 2012
TthPaaR1	Thermus thermophilus			1.1 × 10−3	6 × 10−2		Sakamoto et al., 2011
TthPaaR2				9 × 10−2	5.4 × 10−2