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. 2022 Sep 8;13:975632. doi: 10.3389/fmicb.2022.975632

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Copyright © 2022 Sungsuwan, Kadkanklai, Mhuantong, Jongkaewwattana and Jaru-Ampornpan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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pZAPL interacts with PEDV-N. (A,B) Cell lysates from ZAP-KO HEK293T transfected with pCAGGS-pZAPL-HA or pCAGGS-PEDV-N-Myc were mixed as indicated. A co-IP experiment was performed with agarose beads conjugated with anti-Myc- (A) or anti-HA (B) antibodies. Prey proteins were analyzed by western blot with the indicated antibodies. (C) Co-localization of PEDV-N with pZAPL. VeroE6 cells were co-transfected with pCAGGS-PEDV-N and pCAGGS-pZAPL-HA. Confocal microscopy images of the transfected cells were taken at 48 hpt to examine the localization of the indicated proteins (scale bar = 10 μm).