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. 2022 Sep 8;13:975632. doi: 10.3389/fmicb.2022.975632

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Copyright © 2022 Sungsuwan, Kadkanklai, Mhuantong, Jongkaewwattana and Jaru-Ampornpan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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pCoV-Ns reverses the repression of PB2 expression by zinc-finger antiviral protein (ZAP). (A,B) A pCAGGS plasmid expressing Flag-tagged PB2 was co-transfected into HEK293T cells with a plasmid expressing HA-tagged pZAPL (A) or the non-functional ZAP mutant, ZAP (Y108A) (B), and a plasmid encoding the indicated pCoV-N protein. An empty vector plasmid served as a control. At 48 hpt, cells were lysed, and protein expression was analyzed by SDS-PAGE/western blotting. The western blotting results shown are representative of three independent experiments. The relative band intensities of PB2-FLAG and beta-actin under each lane were analyzed using Image Lab software (Bio-Rad Laboratories, CA, United States). Values shown are the means ± SD of three independent measurements (Student’s t-test, **p < 0.005).