FIGURE 8.

Suppression of CPD-porcine epidemic diarrhea virus (PEDV) growth by pZAPL can be reversed by pCoV-Ns. VeroE6 ZAP-KO cells were transfected with the indicated plasmids. At 24 hpt, transfected cells were infected with CPD-PEDV (MOI = 0.001). Supernatants were collected at 48 and 72 hpi to determine viral titers using the TCID₅₀ assay. Expression of the indicated proteins is shown by SDS-PAGE/western blot results below. Values shown are averages ± SEM of three independent experiments (Student’s t-test, *p < 0.05, **p < 0.005).