FIG. 2.
SmcR affects starvation survival and SIMC at low temperature. V. vulnificus C7184 (filled symbols) and DM7 (smcR::Sm) (open symbols) were grown to mid-exponential phase in LB with NaCl (20 g liter−1), the cells were collected by centrifugation (10,000 × g, 10 min), washed in 2M-C and resuspended in 2M-C. Cultures were held statically at 24°C (A) or were allowed to starve for 0 (B; ▿, ▾) or 4 (B; ○, ●) h before being shifted to 4°C. (C) C7184 starved in the presence (open symbols) or absence (closed symbols) of C2. Determination of CFU was performed on DVNSS agar plates. Data are presented as percentages of the initial count (1.1 × 105 to 2.9 × 105 CFU ml−11) and are representative of three independent experiments. Error bars represent the 95% confidence interval.