TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| V. vulnificus | ||
| C7184 | Human wound isolate | 26 |
| DM7 | smcR::Sm, derived from C7184 | This study |
| UTHS-1 | 39 | |
| V. harveyi | ||
| BB170 | luxN::Tn5 (sensor 1− sensor 2+) | 3 |
| BB152 | luxL::Tn5 (AI-1− AI-2+) | 3 |
| E. coli | ||
| BW20767 | Smr (RP4-2 tet:Mu-1 kan::Tn7 integrant) tra+ leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5 uidA(Mlu1):pir+ thi | 22 |
| DH5α | supE44 ΔlacU169 (Φ80lacZΔM15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 12 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZDM15 Tn10(Tetr)] | 5 |
| Plasmids | ||
| pBluescript II SK(+) | Apr, multiple cloning site flanked by T3 and T7 promoters, lac promoter fused to the α peptide of ′LacZ, derived from pUC19 | Stratagene |
| pCAM140 | Smr, Apr, mini-Tn5 gusA in pUT mini-Tn5 Sm/Sp | 38 |
| pCVD442 | Apr, positive selection vector, pGP704 with sacB inserted in multiple cloning site | 7 |
| pLG401 | Cmr, pACYC Ori, Mob, promoterless gfp with multiple cloning site | Lynn Gilson, University of Hawaii |
| pMacSB | Cmr, sacB from pCVD442 inserted as PstI-EcoRV fragment into cloning site of pLG401 | This study |
| pMacSmcRK | pMacSB with disrupted smcR fragment from pSmcR.SM inserted into EcoRV site of pMacSB | This study |
| pUC19 | Apr, multiple cloning site, lac promoter fused to the α peptide of ′LacZ | 40 |
| pSmcR8 | Apr, pUC19 with 720-bp insert containing V. vulnificus smcR | This study |
| pSmcR8.18 | Apr, pBluescript with ∼770-bp insert containing the EcoRI-HindIII smcR fragment from pSmcR8 | This study |
| pSmcR.SM | Apr, Smr, pSmcR8.18 with ∼2.0-kb Sm fragment from pCAM140 inserted into the BglII site of the smcR insert | This study |