TABLE 2.

Selected phenotypes of a V. vulnificus smcR mutant

V. vulnificus strain	Signal productiona	Alkaline phosphataseb	Biofilm formationc	Protease activityd
DM7 (smcR)	+	230	507.4	121.4
C7184 (smcR+)	+	100	100.0	100
C7184 + C2e	+	NDf	ND	0.001

^aCell-free supernatants were collected from early-stationary-phase cultures, and their ability to induce luminescence in the V. harveyi reporter strain was tested. 

^bAlkaline phosphatase production as percentage of wild-type activity. 

^cBiofilm formation at 24 h in microtiter plates, presented as percentage of wild-type attachment. 

^dProtease production as percentage of wild-type HPA activity. 

^eC2 was added at a concentration of 10 μg ml⁻¹ during mid-exponential phase, and the culture was incubated at 37°C for 4 h (stationary phase) prior to collection of supernatants 

^fND, not determined.