Table 3.
Analysis of rapid screening techniques used in aflatoxin testing.
| No. | Type | Characteristics of analytical method | References |
|---|---|---|---|
| 1. | Enzyme-Linked Immunosorbent Assay (ELISA) | - Most commonly used antibody-based assay currently. | (24) |
| - High specificity | |||
| - Rapid detection | |||
| - Simple design | |||
| - Cheap | |||
| 2. | Lateral Flow Immunoassay (LFIA) | - Rapid detection | (24, 31, 32) |
| - Simple operational method | |||
| 3. | Aptamer-based lateral flow assay | - Greater resistance toward harsh environments compared to lateral flow immunoassay | (24, 33) |
| - Higher sensitivity | |||
| - Higher specificity | |||
| - Preferable for use in complicated matrices | |||
| - Rapid detection | |||
| 4. | Cytometric Bead Array | - New technology | (24, 34) |
| - Early stage | |||
| - Good sensitivity | |||
| - Rapid detection | |||
| - Real-time analysis for trace small molecules (aflatoxins) | |||
| - Suitable for on-site high-throughput detection | |||
| - High cost | |||
| - Complex instruments required |