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PO stimulation causes the down-regulation on the phosphorylated AMPK protein level in primary chicken hepatocytes. The primary chicken hepatocytes were stimulated with 0.75 mmol PO for 0–48 h. (A) Immunoblot of phosphorylated AMP-activated protein kinase α (AMPKα) (Thr172) and AMPKα in primary chicken hepatocytes; (B) Statistical analysis of phospho (p)-AMPKα (Thr172) and AMPKα protein level. Values represent the means ± SE. *p < 0.05 and **p < 0.01, compared with the control group.
