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. 2022 Sep 8;13:974825. doi: 10.3389/fphys.2022.974825

FIGURE 4.

FIGURE 4

AMPK regulates the lipid synthesis related factors expression level in PO-stimulated primary chicken hepatocytes. The primary chicken hepatocytes were pre-treated with/without 10 μM AMPK inhibitor compound C for 1 h, and then stimulated with/without 0.75 mmol PO for another 24 h. (A) Immunoblot of phosphorylated AMP-activated protein kinase α (AMPKα) (Thr172), AMPKα, phospho (p)-acetyl-CoA carboxylase α (p-ACCα), ACCα and fatty acid synthase (FASN). (B) Statistical analysis of p-AMPKα (Thr172)/AMPKα; (C) Statistical analysis of p-ACCα/ACCα; (D) Statistical analysis of FASN/β-actin. The primary chicken hepatocytes were pre-treated with/without 1 mM AMPK activator AICAR for 1 h, and then stimulated with/without 0.75 mmol PO for another 24 h. (E) Immunoblot of p-AMPKα (Thr172), AMPKα, p-ACCα, ACCα and FASN. (F) Statistical analysis of p-AMPKα/AMPKα; (G) Statistical analysis of p-ACCα/ACCα; (H) Statistical analysis of FASN/β-actin. Values represent the means ± SE. *p < 0.05 and **p < 0.01, significantly different between the indicated treatment groups.