Skip to main content
. 2022 Sep 8;13:974825. doi: 10.3389/fphys.2022.974825

FIGURE 5.

FIGURE 5

Inhibition of AMPK signal exacerbates the oxidative stress challenged by PO in primary chicken hepatocytes. The primary chicken hepatocytes were pre-treated with/without 10 μM AMPK activator compound C for 1 h, and then stimulated with/without 0.75 mmol PO for another 24 h. (A) Fluorescence photography of reactive oxygen species (ROS) (scale bars, 100 μm) and mitochondrial ROS (mtROS) (scale bars, 100 μm); (B) Statistical analysis of ROS level; (C) Statistical analysis of mtROS level; (D) Fluorescence photography of mitochondrial membrane potential (MMP) were detected by JC-1 (scale bars, 100 μm); (E) Statistical analysis of MMP; (F) Malondialdehyde (MDA) content; (G) total antioxidant capacity (T-AOC); (H) Superoxide dismutase (SOD) activity; (I) Catalase (CAT) activity; (J) Peroxidase (POD) activity. Values represent the means ± SE. *p < 0.05 and **p < 0.01, significantly different between the indicated treatment groups.