TABLE 5.
List of tentatively identified compounds in the pressurized choline chloride/citric acid/water (1:1:11) extracts of soy branches (B), leaves (L), pods (P), roots (R), and soybeans (S) by UHPLC-ESI-QToF-MS/MS.
| Peak number | Rt (min) | Tentative identification | Classification | Molecular formula | Molecular Ion | Measured mass (Δ ppm) | MS/MS fragments (relative abundance) | B | L | P | R | S | Ref. |
| 1 | 4.18 | Licoagroside B | Glycoside | C18H24O12 | [M+H]+ | 433.1340 (3.8) | 127.0380 (100), 85.0280 (7.8), 128.0420 (6.9) | X | X | X | X | X | |
| 2 | 5.06 | Genistin | Isoflavonoids | C21H20O10 | [M+H]+ | 433.1129 (-3.9) | 255.0650 (100), 256.0680 (19.1), 199.0740 (9.1) | X | X | X | X | X | |
| 3 | 5.26 | Daidzin | Isoflavonoids | C21H20O9 | [M+H]+ | 417.1180 (-0.4) | 255.0670 (100), 256.0660 (25.2), 257.0700 (6.3) | X | X | X | X | X | |
| [M+FA-H]- | 461.1089 (-4.5) | 253.0490 (100), 252.0420 (61.1), 44.9990 (56.7) | |||||||||||
| 4 | 5.30 | Kaempferol 3-rutinoside 4′-glucoside | Flavonoids | C33H40O20 | [M+H]+ | 757.2186 (6.6) | 287.0540 (100), 85.0270 (28.6), 288.0570 (21.3) | X | X | ||||
| 5 | 5.36 | 3-{[(2S,3R,4S,5S,6R)-4,5-Dihydroxy-3-{[3,4,5-trihydroxy-6-(hydroxymethyl) oxan-2-yl]oxy}-6-({[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy}methyl) oxan-2-yl]oxy}-5,7-dihydroxy-2-(4-hydroxyphenyl) chromen-4-one | Flavonoids | C33H40O20 | [M+H]+ | 757.2185 (3.8) | 287.0550 (100), 288.0590 (20.1), 85.0270 (15.2) | X | X | ||||
| 6 | 5.45 | Biochanin A 7-O-glucoside (astroside) | Isoflavonoids | C22H22O10 | [M+Cl]- | 481.0907 (-1.7) | 283.0570 (100), 268.0370 (38.0), 284.0620 (17.2) | X | X | ||||
| 7 | 5.56 | Glycitin | Isoflavonoids | C22H22O10 | [M+H]+ | 447.1286 (0.2) | 285.0746 (100), 286.0786 (21.8), 448.2425 (8.6) | X | X | X | X | ||
| 8 | 6.50 | Malonyldaidzin | Isoflavonoids | C24H22O12 | [M+H]+ | 503.1184 (4.2) | 255.0647 (100), 70.0657 (20.4), 86.0949 (20.4) | X | X | X | |||
| 9 | 7.10 | 6?-O-Acetyldaidzin | Isoflavonoids | C23H22O10 | [M+H]+ | 459.1286 (3.9) | 255.0649 (100), 70.0658 (12.0), 471.2798 (10.1) | X | |||||
| 10 | 7.26 | Formononetin 7-O-glucoside (ononin) | Isoflavonoids | C22H22O9 | [M+H]+ | 431.1336 (6.9) | 269.0800 (100), 270.0830 (25.6), 254.0560 (11.6) | X | X | X | |||
| 11 | 7.41 | 4′,6-Dimethoxyisoflavone-7-O-.beta.-D-glucopyranoside (wistin) | Isoflavonoids | C23H24O10 | [M+H]+ | 461.1442 (2.9) | 299.0900 (100), 284.0670 (42.6), 300.0930 (19.0) | X | |||||
| 12 | 8.45 | Apigenin | Flavonoids | C15H10O5 | [M-H]- | 269.0455 (-5.8) | 269.0412 (100), 151.0321 (23.4), 223.8405 (21.9) | X | X | X | X | X |