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. 2022 Sep 13;11(9):bio059469. doi: 10.1242/bio.059469

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — EV71 co-distributes with RAB9 during the uncoating period. Co-localization of EV71 with RAB5, RAB7, or RAB9 is shown by immunofluorescence of EV71 and RAB5 (A), RAB7 (B), or RAB9 (C). RD-hSCARB2 cells were infected with EV71 and fixed 30 min after infection, followed by immunofluorescence. DAPI is in blue, EV71 antigen is in green, whereas marker antigens are in red. The panels on the right are enlarged 3D cross-section views of the dashed rectangles in the merged panels. Arrowheads indicate co-localization of EV71 with markers. Representative images are shown. Scale bars: 10 µm. (D) Kinetics of co-localization of EV71 with the different markers. Times indicate the time after infection.