Fig. 7.

CD-M6PR is essential for EV71 replication. (A) Western blotting of RAB5, RAB7, RAB9, CD-M6PR, CI-M6PR and clathrin heavy chain in cells in which these genes have been targeted by siRNA knockdown. The expression of β-actin was examined as loading control. Triangles indicate molecular weight markers. Black dots indicate the predicted molecular weight of the proteins. The numbers are the densities and the ratio of anti-markers/anti β-actin. (B) RD cells were treated with siRNAs targeting RAB5, RAB7, RAB9, CD-M6PR, CI-M6PR or clathrin heavy chain and then infected with EV71-GFP. Cells were fixed at 31 h after infection, followed by fluorescent imaging. GFP-positive cells were counted and their percentage against the total number of DAPI-positive cells plotted. *P<0.05. Statistical significance was determined by a two-tailed, unpaired t-test. Error bars represent s.d.