TABLE 5.
Effector | % Activity |
---|---|
None | 100 |
0.1 mM PPi | 20 |
0.1 mM PPi and 0.1 mM ADP | 57 |
0.1 mM PPi and 0.1 mM AMP | 22 |
0.1 mM PPi, 0.1 mM ADP, and 0.1 mM AMP | 57 |
0.1 mM PPi and 0.1 mM TDP | 69 |
0.1 mM PPi and 0.1 mM GDP | 68 |
0.1 mM PPi and 0.1 mM CDP | 27 |
0.1 mM PPi and 0.1 mM UDP | 47 |
Experimental conditions were 0.5 mM F-6-P, 0.25 mM ATP, 5 mM MgCl2, 0.2 mM NADH, 175 mM KCl, and 30 mM Tris (pH 7.8) at 50°C.