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. 2022 Sep 7;27:73–88. doi: 10.1016/j.omtm.2022.09.002

Figure 1.

Figure 1

Establish and validate a transwell model of human BBB for selection of AAVs

(A and B) Permeability of transwells with no cells, hCMEC/D3 or hCMEC/D3 and human primary astrocytes were tested using 70k MW (A) and 2M MW (B) dextran. Significance determined by two-way ANOVA test, ∗∗∗∗p < 0.0001. (C–E) Eighteen uniquely barcoded AAVs were pooled and passaged through the transwell BBB model with hCMEC/D3, primary astrocytes and hiPSC-derived cortical neurons with MOI of 1e4 (C), 1e5 (D), or 1e6 (E). The log2-fold change of the percentage of each AAV in flowthrough media, primary astrocytes with or without Ad5, and in hiPSC-derived cortical neurons with or without Ad5 are graphed in comparison to the percentage in the input.