REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
BrdU (Bu20a) Mouse mAb	Cell signaling Technology	Cat#5292S; RRID: AB_10548898
HIF-1α	Novus Biologicals	Cat#NB100-449; RRID: AB_10001045
HIF-2α	Novus Biologicals	Cat#NB100-122; RRID: AB_10002593
Lamin B1 (D9V6H) Rabbit mAb	Cell signaling Technology	Cat# 13435; RRID: AB_2737428
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)	Abcam	Cat#ab150113; RRID: AB_2576208
Goat anti rabbit Alexa Fluor® 594	Invitrogen	Cat#A32740; RRID: AB_2762824
Goat anti-Rabbit IgG (H + L) Secondary Antibody [HRP]	Novus Biologicals	Cat#NB7160; RRID: AB_524669
Anti-phospho-PDHE1-A type I (Ser293)	Sigma-Aldrich	Cat#ABS204; RRID: AB_11213668
PDH E1 alpha Polyclonal Antibody	Thermo Scientific™	Cat#18068-1-AP; RRID: AB_2162931
Purified anti-GAPDH Antibody	BioLegend	Cat#631402; RRID: AB_2107422
Chemicals, peptides, and recombinant proteins
EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™	Lonza	Cat#CC-3162
Quality Biological Inc Trypsin (0.25%) – EDTA (0.02%)	Thermo Scientific™	Cat#50983216
Dimethyl sulfoxide	Sigma-Aldrich	Cat#D8418
Dimethyloxalylglycine (DMOG)	Cayman Chemical	Cat# 71210
Trimethyl citrate	Sigma-Aldrich	Cat#27502
Methyl pyruvate	Thermo Scientific™	Cat#A13966
N-Methyl-L-aspartic acid	Santa Cruz	sc-212234
Nicotinamide riboside	Cayman Chemical	Cat#23132
Gibco™ DPBS, no calcium, no magnesium	Thermo Scientific™	Cat#14190250
Invitrogen™ Image-iT™ Fixative Solution (4% formaldehyde, methanol-free)	Thermo Scientific™	Cat#R37814
UltraPure™ DNase/RNase-Free Distilled Water	Thermo Scientific™	Cat#10977023
Sodium dichloroacetate (DCA)	Sigma-Aldrich	Cat#347795
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)	Thermo Scientific™	Cat#M6494
BD Bromodeoxyuridine (BrdU)	Thermo Scientific™	Cat#BDB550891
Vectashield Vibrance Antifade Mounting Medium with DAPI - 10 mL	Thermo Scientific™	Cat#NC1601055
PureLink™ RNase A	Thermo Scientific™	Cat#12091021
Propidium Iodide	Thermo Scientific™	Cat# P3566
Thermo Scientific™ RIPA Lysis and Extraction Buffer	Thermo Scientific™	Cat#PI89901
Pierce™ Protease Inhibitor Mini Tablets, EDTA-free	Thermo Scientific™	Cat#A32955
Pierce™ Phosphatase Inhibitor Mini Tablets	Thermo Scientific™	Cat#A32957
NuPAGE™ LDS Sample Buffer (4X)	Thermo Scientific™	Cat#NP0007
Novex™ Tris-Glycine SDS Running Buffer (10X)	Thermo Scientific™	LC2675
Novex™ Tris-Glycine Transfer Buffer (25X)	Thermo Scientific™	Cat#LC3675
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa	Thermo Scientific™	Cat#26619
Methanol (Certified ACS), Fisher Chemical™	Thermo Scientific™	A4124
SuperSignal™ West Femto Maximum Sensitivity Substrate	Thermo Scientific™	Cat#34095
TWEEN® 20	Sigma-Aldrich	Cat#P9416-100ML
Acetonitrile (LC-MS)	MilliporeSigma	Cat# 1000294000
Water, BAKER ANALYZED™ LC/MS Reagent Grade	J.T. Baker	Cat#9831-02
Critical commercial assays
Angiogenesis Assay Kit (In Vitro)	Abcam	Cat#ab204726
Human NADH dehydrogenase ELISA Kit (Complex I)	Abcam	Cat#ab178011
Complex I Enzyme Activity Microplate Assay Kit (Colorimetric)	Abcam	Cat#ab109721
Pierce™ Rapid Gold BCA Protein Assay Kit	Thermo Scientific™	Cat# A53226
Deposited data
RNAseq data	This manuscript-GEO	GSE189504
Experimental models: Cell lines
Primary Pulmonary Artery Endothelial Cells; Normal, Human (HPAEC)	ATCC	Cat# PCS-100-022
Software and algorithms
Prism	Graphpad	https://www.graphpad.com/scientific-software/prism/
FIJI ImageJ	NIH	https://imagej.net/software/fiji/downloads
FastQC	Babraham Bioinformatics	http://www.bioinformatics.babraham.ac.uk/projects/fastqc/
Adobe Illustrator	Adobe	https://www.adobe.com/products/illustrator.html
edgeR	Bioconductor	http://bioconductor.org/packages/release/bioc/html/edgeR.html
Xcalibur 4.1	Thermo Fisher Scientific	https://assets.thermofisher.com/TFS-Assets/CMD/manuals/man-xcali-97928-xcalibur-41-quan-start-manxcali97928-en.pdf
TraceFinder 4.1	Thermo Fisher Scientific	https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/lc-ms-data-acquisition-software/tracefinder-software.html
Other
Western Blotting Filter Paper, Extra Thick, 7 cm × 8.4 cm	Thermo Scientific™	Cat#88605
Novex™ WedgeWell™ 6%, Tris-Glycine, 1.0 mm, Mini Protein Gels	Thermo Scientific™	Cat#XP00060BOX
Novex™ WedgeWell™ 6%, Tris-Glycine, 1.0 mm, Mini Protein Gels	Thermo Scientific™	Cat#XP00062BOX
Novex WedgeWell 4 to 20%, Tris-Glycine, 1.0 mm, Mini Protein Gel, 10-well	Thermo Scientific™	Cat#XP04200BOX
Novex WedgeWell 4 to 20%, Tris-Glycine, 1.0 mm, Mini Protein Gel, 12-well	Thermo Scientific™	Cat#XP04202BOX
Nitrocellulose Membrane, 0.45 μm	Bio-rad	Cat#1620115