KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| ||
| Rabbit anti-eIF5A | Abcam | Cat# ab32443; RRID: AB_2230904 |
| Rabbit anti-Hypusine | Raghu Mirmira Lab | N/A |
| Rabbit anti-Hypusine | Millipore | Cat# ABS1064; RRID: AB_2631138 |
| Rat anti-LANA | Advanced Biotechnologies | Cat# 13-210-100; RRID: AB_1929223 |
| Mouse anti-vIRF3 | Novus | Cat# CM-A807 |
| Rabbit anti-EBNA | Paul Lieberman Lab | N/A |
| Rabbit anti-Flag | Sigma | Cat# F7425; RRID: AB_439687 |
| Mouse anti-β-actin | Santa Cruz | Cat# sc-47778; RRID: AB_626632 |
| Horse Anti-mouse IgG-HRP | Cell Signaling | Cat# 7076; RRID: AB_330924 |
| Goat Anti-rabbit IgG-HRP | Cell Signaling | Cat# 7074; RRID: AB_2099233 |
| Goat Anti-rat IgG-HRP | Santa Cruz | Cat# sc-2006; RRID: AB_112521 |
| Goat anti-Rat IgG-Alexa Fluor 568 | Thermo Fisher | Cat# A-11077; RRID: AB_2534121 |
| Mouse anti-GAPDH | Santa Cruz | Cat# sc-47724; RRID: AB_627678 |
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| Bacterial and virus strains | ||
|
| ||
| BAC16 KSHV | Brulois et al., 2012 | N/A |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Spermidine | Cayman Chemical | 14918 |
| Doxycycline hyclate | Sigma | D9891 |
| Sodium butyrate | Sigma | 303410 |
| Novex™ pH 3-7 IEF Protein Gels, 1.0 mm, 10-well |
ThermoFisher | EC6645BOX |
| Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free (Matrigel) |
Corning | 356231 |
| FITC-Annexin V | Biolegend | 640906 |
| Propidium Iodide | Biolegend | 421301 |
| TRIzol | Thermo Fisher | 15596018 |
| iScript™ cDNA Synthesis Kit | Bio-Rad | 1708891BUN |
| SsoAdvanced Universal SYBR Green Supermix | Bio-Rad | 1725272 |
| GC7 | Millipore | 259545 |
| DFMO | Cayman Chemical | 16899 |
| D-Luciferin, Potassium Salt | Goldbio | LUCK-100 |
| Poly-L-lysine solution | Sigma | P4707 |
| CleanCap AG | Trilink | N-7413-5 |
| E. coli Poly(A) Polymerase | New England Biolabs | M0276 |
| Methanol, LC/MS Grade | Fisher Chemical | A456 |
| Acetonitrile, LC/MS Grade | Fisher Chemical | A955 |
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| Critical commercial assays | ||
|
| ||
| CellTiter-Glo 2.0 Assay | Promega | G9241 |
| MasterPure Complete DNA and RNA Purification Kit | Lucigen | MC85200 |
| HiScribe T7 High Yield RNA Synthesis Kit | New England Biolabs | E2040S |
| RNA Clean & Concentrator-5 | Zymo Research | R-1014 |
|
| ||
| Deposited data | ||
|
| ||
| Normalized mass spectrometry metabolomics data | This paper | Table S1 |
| Raw mass spectrometry metabolomics data | This paper | MTBLS5218 |
|
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| Experimental models: Cell lines | ||
|
| ||
| Human: Embryonic kidney 293T cells | ATCC | CRL-3216 |
| Human: iSLK cells | Myoung J et al., 2011 | N/A |
| Human: iSLK-BAC16 cells | Brulois et al., 2012 | N/A |
| Human: BCBL-1 | Donald Ganem Lab | N/A |
| Human: BCBL-1-luciferase | Lee et al., 2011 | N/A |
| Human: BC-1 | ATCC | CRL-2230 |
| Human: BC-3 | ATCC | CRL-2277 |
| Human: BJAB | DSMZ | ACC 757 |
| Human: BEL-1 | Tang et al.,2004 | N/A |
| Human: BV173 | DSMZ | ACC 20 |
| Human: BJAB-luciferase | This paper | N/A |
| Human: LCL352-mCherry-luciferase | Paul M. Lieberman Lab | N/A |
| Human: Endothelial cells immortalized with hTERT (TIME) | ATCC | CRL-4025 |
| Human: Mammary gland epithelial cells (MCF10A) | ATCC | CRL-10317 |
|
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| Experimental models: Organisms/strains | ||
|
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| Mouse: NOD/SCID | The Jackson Laboratory | 001303 |
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| Oligonucleotides | ||
|
| ||
| Primer for qPCR (See Table S2) | Integrated DNA Technologies | N/A |
|
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| Recombinant DNA | ||
|
| ||
| pCDH-puro-LANA | This paper | N/A |
| pCDH-puro-LANA (N) | This paper | N/A |
| pCDH-puro-LANA (A) | This paper | N/A |
| pCDH-puro-LANA (C) | This paper | N/A |
| pCDH-puro-vIRF3 | This paper | N/A |
| pCDH-puro-EBNA1 | This paper | N/A |
| pCXWB-EBNA1 | Addgene | Plasmid #37624 |
| pZMV plasmid | This paper | N/A |
| pZMV-LANA | This paper | N/A |
| pZMV-vIRF3 | This paper | N/A |
| pZMV-EBNA1 | This paper | N/A |
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| Software and algorithms | ||
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| ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Prism 9 | GraphPad Software | N/A |
| Agilent Qualitative Analysis B.07.00 | Agilent Technologies | N/A |
| Profinder B.08.00 | Agilent Technologies | N/A |
| FlowJo | BD | N/A |